Wounding may be the primary external trigger for callus induction from explants, that is the effect of a group of damage-associated molecular patterns. These local wounding signals are translated into long-distance signals like the electrical signal of glutamate receptor-like cation channels, thereby inducing epigenetic modifications, alterations in the synthesis and accumulation of cytokinin and free auxin, and transcriptional upregulation of growth and developmental regulatory genes. Transcriptional changes are the activated expression of callus-inductive chromatin-remodeling regulator genes. These regeneration-promoting factors interact to tightly and precisely control each step of callus formation and plant regeneration.
How far better use plant developmental regulatory genes in plant transformation is really a challenging question. Constitutive or ectopic expression of all such genes typically inhibits normal plant growth and development and causes undesirable pleiotropic effects. Fine-tuning the expression of the genes is crucial for the regeneration of normal, fertile plants of different species. The researchers summarized advantages and disadvantages of the regulatory factors and discussed the usage of a dexamethasone (Dex)-inducible expression system or estradiol-inducible expression system to fine-tune their expression.
The review was published in the journal Horticulture Research.
More info: Nathan A Maren et al, Genotype-independent plant transformation, Horticulture Research (2022). DOI: 10.1093/hr/uhac047
Provided byNanjing Agricultural University The Academy of Science
Citation: An assessment article clarifies genotype-independent plant transformation (2022, August 12) retrieved 14 August 2022 from https://phys.org/news/2022-08-article-genotype-independent.html
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